Kinetics and Thermodynamic Study of Urease Extracted from Soybeans
نویسندگان
چکیده
The present study is concerned with the kinetics and thermodynamic study of urease extracted from soybeans. The effect of pH, temperature, concentration of substrate and incubation time on the urease activity was examined. Maximum urease activity (86.79 ± 2.43 U/g) was obtained at 30 o C and pH 8.0 after 60 sec of incubation. Kinetics constants i.e. Km and Vmax were estimated by using Lineweaver-Burk plot and were found to be 36.49 mM and 87.72 U/g, respectively. Thermodynamic constants i.e. Activation energy (Ea), change in enthalpy (ΔH) and change in entropy (ΔS) were calculated using Arrhenius plot and were found to be 5.7 KJ/mol, 3.468 KJ/mol and -4.57 KJ/mol respectively.
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